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Forward Primer (10 μM)
so 4°C is preferred
Optimization methods: ① Rinse the inner wall of the centrifuge tube with PBS containing 5% FBS before rinsing to form a protective film and reduce cell adhesion
Optimized conditions for incubation time
FireGene Gastrointestinal Dissociation Kit - Gut Cell Isolation Option:10 reactions/kit Forward Primer (10 μM)Overview FireGene Gastrointestinal Dissociation Kit is a high performance enzymatic solution designed to isolate viable single cell suspensions from GI tissues. Optimized for applications like single cell sequencing, immune profiling, and gut microbiota research, this kit delivers exceptional results for complex and fragile gastrointestinal samples. Background Information Driven by Clinical and Scientific Research Needs: Single cell sequencing of GI